Most read papers

The most frequently downloaded papers for the 12 months to the end of July 2010 are shown below

10382	Biological variability in the structures of diphosphoinositol polyphosphates in Dictyostelium discoideum and mammalian cells C Albert, S T Safrany, M E Bembenek, K M Reddy, K Reddy, J Falck, M Bröcker, S B Shears and G W Mayr (1997) 327, 553-560 Abstract | Legacy HTML | Full text PDF
9214	A sodium carbonate-bicarbonate buffer for alkaline phosphatases G E Delory (1945) 39, 245-245 Abstract | Full text PDF
7426	Ability of a beta-casein phosphopeptide to modulate the precipitation of calcium phosphate by forming amorphous dicalcium phosphate nanoclusters C Holt, N M Wahlgren and T Drakenberg (1996) 314, 1035-1039 Abstract | Legacy HTML | Full text PDF
5921	The selective fermentation of glucose and fructose by yeast E R Dawson (1932) 26, 531-535 Abstract | Full text PDF
5539	Protein S-thiolation targets glycolysis and protein synthesis in response to oxidative stress in the yeast Saccharomyces cerevisiae Daniel Shenton and Chris M Grant (2003) 374, 513-519 doi:10.1042/BJ20030414 Abstract | Legacy HTML | Full text PDF
5318	Specificity and mechanism of action of some commonly used protein kinase inhibitors S P Davies, H Reddy, M Caivano and P Cohen (2000) 351, 95-105 Abstract | Legacy HTML | Full text PDF
5275	Glutamine synthesis is heterogeneous and differentially regulated along the rabbit renal proximal tubule B Ferrier, A Conjard, M Martin and G Baverel (1999) 337, 543-550 Abstract | Legacy HTML | Full text PDF
4758	Modulation of circulatory residence of recombinant acetylcholinesterase through biochemical or genetic manipulation of sialylation levels T Chitlaru, C Kronman, M Zeevi, M Kam, A Harel, A Ordentlich, B Velan and A Shafferman (1998) 336, 647-658 Abstract | Legacy HTML | Full text PDF
4428	Protein kinase B/Akt is essential for the insulin- but not progesterone-stimulated resumption of meiosis in Xenopus oocytes Carsten B Andersen, Hiroshi Sakaue, Taku Nedachi, Kristina S Kovacina, Carol Clayberger, Marco Conti and Richard A Roth (2003) 369, 227-238 doi:10.1042/BJ20021243 Abstract | Legacy HTML | Full text PDF
4261	Activation of nuclear factor-kappaB during doxorubicin-induced apoptosis in endothelial cells and myocytes is pro-apoptotic: the role of hydrogen peroxide Suwei Wang, Srigiridhar Kotamraju, Eugene Konorev, Shasi Kalivendi, Joy Joseph and Balaraman Kalyanaraman (2002) 367, 729-740 doi:10.1042/BJ20020752 Abstract | Legacy HTML | Full text PDF
3946	High-throughput metabolic state analysis: the missing link in integrated functional genomics of yeasts Silas G Villas-Bôas, Joel F Moxley, Mats Akesson, Gregory Stephanopoulos and Jens Nielsen (2005) 388, 669-677 doi:10.1042/BJ20041162 Abstract | Enhanced Full Text | Legacy HTML | Full text PDF
3916	Differential signalling by muscarinic receptors in smooth muscle: m2-mediated inactivation of myosin light chain kinase via Gi3, Cdc42/Rac1 and p21-activated kinase 1 pathway, and m3-mediated MLC20 (20 kDa regulatory light chain of myosin II) phosphorylation via Rho-associated kinase/myosin phosphatase targeting subunit 1 and protein kinase C/CPI-17 pathway Karnam S Murthy, Huiping Zhou, John R Grider, David L Brautigan, Masumi Eto and Gabriel M Makhlouf (2003) 374, 145-155 doi:10.1042/BJ20021274 Abstract | Legacy HTML | Full text PDF
3863	Nucling mediates apoptosis by inhibiting expression of galectin-3 through interference with nuclear factor kappaB signalling Li Liu, Takashi Sakai, Nobuya Sano and Kiyoshi Fukui (2004) 380, 31-41 doi:10.1042/BJ20031300 Abstract | Legacy HTML | Full text PDF
3775	Conserved arginine-516 of Penicillium amagasakiense glucose oxidase is essential for the efficient binding of beta-D-glucose S Witt, G Wohlfahrt, D Schomburg, H J Hecht and H M Kalisz (2000) 347, 553-559 Abstract | Legacy HTML | Full text PDF
3451	Identification of an octamer element required for in vivo expression of the TIE1 gene in endothelial cells S C Boutet, T Quertermous and B M Fadel (2001) 360, 23-29 Abstract | Legacy HTML | Full text PDF
3426	The ninhydrin reaction and its analytical applications H MEYER (1957) 67, 333-340 Abstract | Full text PDF
3327	The estimation of carbohydrates in plant extracts by anthrone E W YEMM and A J WILLIS (1954) 57, 508-514 Abstract | Full text PDF
3278	The determination of cholesterol by the Liebermann-Burchard reaction A P KENNY (1952) 52, 611-619 Abstract | Full text PDF
3204	Overexpression of UDP-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of K5 polysaccharide Elisabet Roman, Ian Roberts, Kerstin Lidholt and Marion Kusche-Gullberg (2003) 374, 767-772 doi:10.1042/BJ20030365 Abstract | Legacy HTML | Full text PDF
3204	Purification and characterization of autophagosomes from rat hepatocytes P E Strømhaug, T O Berg, M Fengsrud and P O Seglen (1998) 335, 217-224 Abstract | Legacy HTML | Full text PDF
3062	Estimation of the molecular weights of proteins by Sephadex gel-filtration P Andrews (1964) 91, 222-233 Abstract | Full text PDF
2885	Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway Jin Lee, Andrew Shirk, John F Oram, Sum P Lee and Rahul Kuver (2002) 364, 475-484 doi:10.1042/BJ20011493 Abstract | Legacy HTML | Full text PDF
2877	Different kynurenine pathway enzymes limit quinolinic acid formation by various human cell types M P Heyes, C Y Chen, E O Major and K Saito (1997) 326, 351-356 Abstract | Legacy HTML | Full text PDF
2791	Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea Aglaia Pappa, Tia Estey, Rizwan Manzer, Donald Brown and Vasilis Vasiliou (2003) 376, 615-623 doi:10.1042/BJ20030810 Abstract | Legacy HTML | Full text PDF
2741	Formation of glyoxal, methylglyoxal and 3-deoxyglucosone in the glycation of proteins by glucose P J Thornalley, A Langborg and H S Minhas (1999) 344, 109-116 Abstract | Legacy HTML | Full text PDF
2726	G-protein coupling of mu-opioid receptors (OP3): elevated basal signalling activity N T Burford, D Wang and W Sadée (2000) 348, 531-537 Abstract | Legacy HTML | Full text PDF
2685	The selectivity of protein kinase inhibitors; a further update Jenny Bain, Lorna Plater, Matt Elliott, Natalia Shpiro, James Hastie, Hilary McLauchlan, Iva Klevernic, Simon Arthur, Dario Alessi and Philip Cohen (2007) 408, 297-315 doi:10.1042/BJ20070797 Abstract | Enhanced Full Text | Legacy HTML | Full text PDF
2602	Solvent kinetic isotope effects of human placental alkaline phosphatase in reverse micelles T M Huang, H C Hung, T C Chang and G G Chang (1998) 330, 267-275 Abstract | Legacy HTML | Full text PDF
2591	Colorimetric determination of potassium by Folin-Ciocalteu phenol reagent M A M ABDUL-FADL (1949) 44, 282-285 Abstract | Full text PDF
2545	A photoelectric flame photometer W R DOMINGO and W KLYNE (1949) 45, 400-408 Abstract | Full text PDF
2525	Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli S Kajiwara, P D Fraser, K Kondo and N Misawa (1997) 324, 421-426 Abstract | Legacy HTML | Full text PDF
2478	Mutational analysis of the catalytic centre of the Citrobacter freundii AmpD N-acetylmuramyl-L-alanine amidase Catherine Généreux, Dominique Dehareng, Bart Devreese, Jozef Van Beeumen, Jean-Marie Frère and Bernard Joris (2004) 377, 111-120 doi:10.1042/BJ20030862 Abstract | Legacy HTML | Full text PDF
2466	Intracellular metabolism and bioactivity of quercetin and its in vivo metabolites Jeremy P E Spencer, Gunter G C Kuhnle, Robert J Williams and Catherine Rice-Evans (2003) 372, 173-181 doi:10.1042/BJ20021972 Abstract | Legacy HTML | Full text PDF
2430	The use of non-aqueous chloroform/methanol extraction for the delipidation of brain with minimal loss of enzyme activities T Kurihara, Y Nishizawa and Y Takahashi (1977) 165, 135-140 Abstract | Full text PDF
2412	A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid K BURTON (1956) 62, 315-323 Abstract | Full text PDF
2406	Partial reconstruction of in vitro gluconeogenesis arising from mitochondrial l-lactate uptake/metabolism and oxaloacetate export via novel L-lactate translocators Lidia De Bari, Anna Atlante, Daniela Valenti and Salvatore Passarella (2004) 380, 231-242 doi:10.1042/BJ20031981 Abstract | Legacy HTML | Full text PDF
2403	Control analysis of lipid biosynthesis in tissue cultures from oil crops shows that flux control is shared between fatty acid synthesis and lipid assembly Umi S Ramli, Darren S Baker, Patti A Quant and John L Harwood (2002) 364, 393-401 doi:10.1042/BJ20010203 Abstract | Legacy HTML | Full text PDF
2380	Enzymic formation of riboflavin 4',5'-cyclic phosphate from FAD: evidence for a specific low-Km FMN cyclase in rat liver1 F J Fraiz, R M Pinto, M J Costas, M Aavalos, J Canales, A Cabezas and J C Cameselle (1998) 330, 881-888 Abstract | Legacy HTML | Full text PDF
2305	The specificities of protein kinase inhibitors: an update Jenny Bain, Hilary McLauchlan, Matthew Elliott and Philip Cohen (2003) 371, 199-204 doi:10.1042/BJ20021535 Abstract | Legacy HTML | Full text PDF
2279	A rapid assay for peroxidase activity R P F Gregory (1966) 101, 582-583 Abstract | Full text PDF
2266	Redox regulation of cAMP levels by ascorbate in 1,25-dihydroxy- vitamin D3-induced differentiation of HL-60 cells G López-Lluch, M I Burón, F J Alcaín, J M Quesada and P Navas (1998) 331, 21-27 Abstract | Legacy HTML | Full text PDF
2260	myo-Inositol 1,4,5-trisphosphate and Ca(2+)/calmodulin-dependent factors mediate transduction of compression-induced signals in bovine articular chondrocytes Wilmot B Valhmu and Frank J Raia (2002) 361, 689-696 Abstract | Legacy HTML | Full text PDF
2224	DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides S Dowler, R A Currie, C P Downes and D R Alessi (1999) 342, 7-12 Abstract | Legacy HTML | Full text PDF
2209	Binding of the merlin-I product of the neurofibromatosis type 2 tumour suppressor gene to a novel site in beta-fodrin is regulated by association between merlin domains G W Neill and M R Crompton (2001) 358, 727-735 Abstract | Legacy HTML | Full text PDF
2200	Mevalonate-derived isopentenyl diphosphate is the biosynthetic precursor of ubiquinone prenyl side chain in tobacco BY-2 cells A Disch, A Hemmerlin, T J Bach and M Rohmer (1998) 331, 615-621 Abstract | Legacy HTML | Full text PDF
2111	The Viscosity of Blood J W Trevan (1918) 12, 60-71 Abstract | Full text PDF
2097	Inactivation of cysteine proteases by peptidyl epoxides: characterization of the alkylation sites on the enzyme and the inactivator A Albeck and S Kliper (2000) 346, 71-76 Abstract | Legacy HTML | Full text PDF
2024	Prenylation of Rab8 GTPase by type I and type II geranylgeranyl transferases A L Wilson, R A Erdman, F Castellano and W A Maltese (1998) 333, 497-504 Abstract | Legacy HTML | Full text PDF
2019	Evidence for 'lock and key' character in an anti-phosphonate hydrolytic antibody catalytic site augmented by non-reaction centre recognition: variation in substrate selectivity between an anti-phosphonate antibody, an anti-phosphate antibody and two hydrolytic enzymes Sanjiv Sonkaria, Guillaume Boucher, José Flórez-Olvarez, Bilal Said, Syeed Hussain, Elizabeth L Ostler, Sheraz Gul, Emrys W Thomas, Marina Resmini, Gerard Gallacher and Keith Brocklehurst (2004) 381, 125-130 doi:10.1042/BJ20031966 Abstract | Legacy HTML | Full text PDF
2013	Assay of advanced glycation endproducts (AGEs): surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to Nepsilon-carboxymethyl-lysine- and Nepsilon-(1-carboxyethyl)lysine-modified albumin Naila Ahmed, Ognian K Argirov, Harjit S Minhas, Carlos A A Cordeiro and Paul J Thornalley (2002) 364, 1-14 Abstract | Legacy HTML | Full text PDF